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Human Androgen (Androgen) ELISA Kit

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Product Name:

Human Androgen (Androgen) ELISA Kit

Cat.#:

JL22947

Regulatory Status:
Alternative:

Androgen

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
62.5-4000pg/mL
Sensitivity:
31.8pg/mL
Specificity:
This kit recognizes Human Androgen in samples. No significant cross-reactivity or interference between Human Androgen and analogues was observed.
Sample Type:
Serum;plasma and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Metabolism
  • Human Androgen (Androgen) ELISA Kit
  • Human Androgen (Androgen) ELISA Kit
  • Human Androgen (Androgen) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| Androgen

An androgen is any natural or synthetic steroid hormone that regulates the development and maintenance of male characteristics in vertebrates by binding to androgen receptors.This includes the embryological development of the primary male sex organs, and the development of male secondary sex characteristics at puberty. Androgens are synthesized in the testes, the ovaries, and the adrenal glands.Although androgens are commonly thought of only as male sex hormones, females also have them, but at lower levels: they function in libido and sexual arousal. Androgens are the precursors to estrogens in both men and women.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (pg/mL) 4000 2000 1000 500 250 125 62.5 0
OD 0.216 0.298 0.417 0.669 0.955 1.405 1.79 1.865
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human Androgen spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 81-101 96
Plasma(n=8) 92-105 101





 

| Linearity

Samples were spiked with high concentrations of Human Androgen and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4)
1 : 2 Range (%) 83-95 88-96
Average (%) 91 93
1 : 4 Range (%) 89-104 87-108
Average (%) 94 98







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
400 / 400
96T
1
560 / 560
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